Blueprint Haircare Stack: Product Review

Article Summary

Bryan Johnson’s Blueprint Haircare Stack promises hair longevity rooted in science, but how much does that translate into real-world results? In this review, we unpack the ingredients, scrutinize the evidence, compare it to proven alternatives, and ask whether the Haircare Stack offers meaningful benefits beyond its branding and conceptual appeal.

Full Article

Bryan Johnson has made headlines in recent years for his ongoing efforts to combat aging, which he has branded ‘Project Blueprint’. If you’ve seen the Netflix documentary Don’t Die: The Man Who Wants to Live Forever, you’ll be aware of his commitment to anti-aging, as well as his sometimes controversial methods. 

Project Blueprint now encompasses a range of anti-aging supplements sold under the Blueprint banner, including nutritional supplements, skin care products, and the Blueprint Hair Stack. Blueprint products are marketed as an extension of Johnson’s own personal brand, emphasizing high-tech, biometric longevity with a focus on science-backed formulations and lifestyle optimization. 

The stack contains a serum and a shampoo, and claims to help improve the appearance of the scalp and hair. “This is hair longevity rooted in science”, the Blueprint website states. In this article, we will examine that claim. We’ll take a closer look at the formulation’s ingredients, the scientific evidence supporting their use for hair loss, and how it compares to competitors.

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Key Takeaways

• Branding – Blueprint positions its Haircare Stack as a high-tech, longevity-focused solution aligned with Bryan Johnson’s broader biohacking philosophy. The aesthetic is sleek, biotech-driven, and targeted toward wellness-tech consumers rather than traditional dermatology audiences.
• Unique Selling Point – The products emphasize synthetic human peptides, nanoliposome delivery, and longevity-inspired ingredients like NMN and curcumin.
• Clinical Support – No clinical trials have been conducted on the finished serum or shampoo. Ingredient-level evidence is mainly limited to in vitro, animal, or low-quality human studies, with only minor support for caffeine and adenosine in improving shedding or hair thickness.
• Concerns – The formula omits well-studied hair-loss ingredients, instead favoring mechanistically intriguing but poorly validated molecules. Many peptides lack any human data, and concentrations of key actives are undisclosed.
• Evidence Quality – The Blueprint Haircare Stack scored 9/100 for evidence quality, by our metrics.
• Recommendations to Blueprint – Conduct product-specific clinical trials with objective endpoints and disclose active concentrations to enhance transparency. Consider adding at least one evidence-based ingredient and aligning marketing language with available data to avoid overstating efficacy.

What is Blueprint?

Blueprint as a brand stemmed from Bryan Johnson’s own Blueprint project, an ambitious and wide-reaching program of self-experimentation and self-optimization with one primary goal: ‘Don’t Die’. While his stated aim might be hyperbolic, he has found some fame and a sizable following through his endeavors to find lifestyle ‘protocols’ that can slow the aging process and help him maintain and improve his physical health.

Blueprint sells a range of products that could aid in this struggle against aging. Best sellers include the Longevity Mix drink supplement and antioxidant capsules. Blueprint Haircare is consistent with this overall philosophy and is marketed to provide ‘hair longevity rooted in science’.

Who is their Target Consumer?

Blueprint branding and marketing position it firmly within the world of health and wellness tech, which focuses on quantifiable metrics and optimization. Bryan Johnson himself typifies this approach: he has been described as ‘the most measured man alive’ and collects comprehensive biometric data about himself daily. His approach to wellness is holistic, and he catalogs his health-maximized lifestyle in detail, emphasizing ‘science-backed’ solutions. Even the ‘stack’ branding is borrowed from the tech world.

These brands tend to appeal to younger people, particularly millennials and Gen Z. They are typically interested in optimizing their health and time; prioritizing the gym and healthy eating as part of a holistic approach to preventive medicine. As such, many products within the wellness tech space are sold on the promise of maintaining health, rather than as a reactive measure once issues have already arisen.

Interestingly, while high-tech brands and biohacking influencers emphasize the science-backed nature of their products and healthcare regimens, they may also reject conventional medication and tried-and-tested solutions in favor of exciting new innovations and non-pharmaceutical interventions.

What is the Haircare Stack?

Blueprint Haircare includes the Peptide Shampoo and Peptide Serum, marketed as synergistic components in a ‘stack’. The marketing for both products emphasizes their potential impact on hair appearance and scalp health. The Blueprint outlines a two-step routine: use the Peptide Shampoo after your normal shampoo when washing your hair, and apply the Peptide Serum to a dry scalp in the morning or at night. The use of the Blueprint Red Light Laser Cap is also recommended. 

The claims for the action of the stack include promoting the appearance of longer-lasting, fuller-looking hair, supporting hair vitality, supporting a healthy scalp, and supporting the scalp barrier.

What Do They Claim is Unique About This Product?

Blueprint emphasizes its use of peptides, in particular synthetic oligopeptides and polypeptides. They also highlight the nanoliposome delivery system, which aims to improve the targeting of these peptides and other ingredients to key areas of the scalp. As with all of the Blueprint range, the product is positioned as a science-backed solution.

Price Point

Blueprint Haircare Stack – $178

Peptide Serum (50ml) – $119

Peptide Shampoo (100ml)  – $59

Blueprint Haircare sits firmly in the premium pricing tier. While the brand justifies this positioning through its use of synthetic peptides, nanoliposomes, and longevity-focused actives, the scientific support behind many of these ingredients remains limited. 

Given the lack of product-specific clinical trials and the modest evidence for several key actives, the ingredient-to-price ratio is difficult to justify for consumers seeking proven hair regrowth. For most users, the premium cost reflects branding and conceptual innovation more than demonstrated efficacy.

Product Science: Deep Dive

As is often the case with natural, non-pharmaceutical treatments, the marketing for the Blueprint Hair Stack does not claim to promote hair growth. Wording is carefully selected to avoid unverified claims, and instead focuses on the appearance of hair, and ‘supporting’ hair and scalp health. 

Here, we’ll take a deep dive into the scientific evidence that might support the use of the ingredients in the Peptide Serum and Shampoo to support hair health. As the product is positioned among a range of longevity treatments, we’ll also take a look at their capacity to slow or reverse hair loss, or promote hair growth. 

Peptide Serum

Peptides are the building blocks of proteins, and you’ll often see products with peptides marketed as containing essential components of a range of biological processes. The Blueprint Hair Stack emphasizes the use of synthetic human peptides, which gives the formulation a high-tech veneer. 

Peptides themselves are made up of amino acids. Oligopeptides typically contain 2 to 20 amino acids, while polypeptides are longer, containing over 20 amino acids. Synthetic oligopeptides and polypeptides are often designed to mimic the effects of natural molecules, particularly the active regions of proteins, hormones, and biological signaling molecules. Peptides that recreate the activity of natural molecules in this way are called biomimetic. These designs allow researchers and manufacturers to target specific biological pathways or interactions.^[2]Groß, A., Hashimoto, C., Sticht, H., & Eichler, J. (2016). Synthetic peptides as protein mimics. *Frontiers in Bioengineering and Biotechnology.* 3. 211. Available at: … Continue reading

However, evidence for the efficacy of topical peptides in hair regrowth is minimal. The story for many of the synthetic peptide ingredients, detailed below, is similar. They are often known to be involved in hair growth and hair follicle development, but there is little clinical evidence to support their use for hair loss.

Full ingredients list:

The full list of ingredients for the Blueprint Peptide Serum is as follows: water, pentylene glycol, glycerin, hydroxyethyl acrylate/sodium acryloyldimethyl taurate, sh-oligopeptide-1, sh-oligopeptide-4, sh-polypeptide-4, sh-polypeptide-7, sh-polypeptide-9, sh-polypeptide-59, sh-polypeptide-86, nicotinamide mononucleotide, curcumin, tetrahydrocurcumin, copper tripeptide-1, panthenol, caffeine, adenosine, niacinamide, arginine, ketoglutaric acid, salicylic acid, thioctic acid, lecithin, 1,2-hexanediol, acetyl cysteine, glycine, 3-O-ethyl ascorbic acid, P-anisic acid, polysorbate 60, sodium citrate, citric acid, glutathione.

First, we’ll take a look at some of the synthetic peptides included in the serum.

SH-Polypeptide-86

The Blueprint website highlights sh-polypeptide-86 as a key ingredient.  Sh-polypeptide-86 is a synthetic peptide used primarily in cosmetic and personal care products for its potential anti-aging and skin-conditioning properties. It is engineered to mimic naturally occurring peptides in human skin, and often products using it aim to enhance skin repair and support collagen production

Sh-polypeptide-86 is sometimes branded as follistatin because it mimics some of the activity of the naturally occurring protein of the same name. No preclinical or clinical data are available on the efficacy of sh-polypeptide-86 in treating hair loss. However, there has been some research into the effectiveness of follistatin. 

Follistatin has inhibitory binding activity: when it binds with certain molecules, it prevents their activity. Some of the molecules it can inhibit include activins and bone morphogenetic proteins (BMPs), which both inhibit hair follicle activation. Therefore, there is a potential mechanism through which follistatin might aid in hair growth.^[3]McDowall, M., Edwards, N. M., Jahoda, C. A. B., & Hynd, P. I. (2008). The role of activins and follistatins in skin and hair follicle development and function. Cytokine & Growth Factor … Continue reading

Animal studies show that follistatin levels are associated with the propagation of “hair waves” – coordinated periods of growth across sets of hair follicles in mice. One research group showed that follistatin can induce these waves, switching on hair growth in patches of fur. Declines in follistatin as mice age can delay and reduce these waves.^[4]Chen, C.-C., Murray, P. J., Jiang, T. X., Plikus, M. V., Chang, Y.-T., Lee, O. K., Widelitz, R. B., & Chuong, C.-M. (2014). Regenerative hair waves in aging mice and extra-follicular modulators … Continue reading 

However, in humans, scalp follicles cycle almost completely out of sync, with each follicle running on its own multi‑year timetable, so there is no visible wave pattern across the scalp. Human follicles are also larger, sit in thicker skin, and are more strongly influenced by hormones such as androgens than mouse pelage is, especially in androgenic alopecia. That means changes in follistatin seen in mouse “hair waves” may still matter for human biology, but the effects are likely to be subtler, slower, and layered on top of many other regulatory signals rather than producing dramatic, synchronized bursts of regrowth.

However, clinical studies in humans are minimal. One phase 1, randomized controlled study assessed the efficacy of a ‘hair-stimulating complex’, which included follistatin alongside a protein called Wnt and the signaling molecules keratinocyte growth factor (KGF) and vascular endothelial growth factor (VEGF).^[5]Zimber, M. P., Ziering, C., Zeigler, F., Hubka, M., Mansbridge, J. N., Baumgartner, M., Hubka, K., Kellar, R., Perez-Meza, D., & Sadick, N. (2011). Hair regrowth following a Wnt- and … Continue reading

Wnt signaling is essentially the master switch for hair follicle formation, regeneration, and cycling, and its loss is tightly linked to hair thinning and androgenic alopecia. Similarly, VEGF plays a vital role in supporting the blood vessel network around cells, which can help hair follicles grow thicker, longer hairs, and prolong the anagen phase.

They found an increase in terminal hair density after a single injection. Notably, the solution was not applied topically. Due to the nature of the mixture of molecules in the hair-stimulating complex, it is not possible to draw any firm conclusions about the efficacy of follistatin from this study. No follow-up studies have been conducted since this study, which was published in 2011.

SH-Oligopeptide-4

The only clinical studies including sh-oligopeptide-4 use the peptide as part of a combination therapy called QR 678, which is injected intradermally. As such, these studies can’t provide any insight into its effect alone when applied topically.^[6]Kapoor, R., Shome, D., Vadera, S., & Ram, M. S. (2020). QR 678 & QR678 Neo vs PRP—A randomized, comparative, prospective study. Journal of Cosmetic Dermatology. 19(11). 2877-2885. Available … Continue reading

However, sh-oligopeptide-4 is a synthetic analog of Thymosin-β4, and its potential activity in hair growth is based on mimicking the regenerative and pro-growth actions of natural Thymosin-β4 in hair follicles. Evidence from animal models suggests that thymosin-β4 stimulates hair follicle stem cells to migrate from the stem cell-rich bulge to the base of the follicle, initiating and supporting the anagen phase.^[7]Philp, D., Nguyen, M., Scheremeta, B., St-Surin, S., Villa, A. M., Orgel, A., Kleinman, H. K., & Elkin, M. (2004). Thymosin β4 increases hair growth by activation of hair follicle stem cells. … Continue reading

Further animal studies have indicated that it also promotes the formation of new blood vessels around hair follicles and may increase hair growth by activating the proteins P38, ERK, and Akt. When you remove Thymosin-β4 through genetic knockouts in mice, hair growth is slower, and the activity of these key proteins is reduced.^[8]Philp, D., Nguyen, M., Scheremeta, B., St-Surin, S., Villa, A. M., Orgel, A., Kleinman, H. K., & Elkin, M. (2004). Thymosin β4 increases hair growth by activation of hair follicle stem cells. … Continue reading,^[9]Gao, X., Liang, H., Hou, F., Zhang, Z., Nuo, M., Guo, X., & Liu, D. (2015). Thymosin beta-4 induces mouse hair growth. *PLOS ONE.* 10(6). e0130040. Available at: … Continue reading

Despite laboratory evidence for a role of Thymosin-β4 in hair follicle activation, there is no clinical evidence supporting its use to promote hair growth or, more generally, hair health.

SH-Oligopeptide-1

While sh-oligopeptide-1 is found in some topical solutions designed to promote skin health, it is not typically used in products that target hair health or regrowth.  The synthetic peptide was designed to mimic human Epidermal Growth Factor (EGF), a naturally occurring protein that plays a central role in regulating cell growth, differentiation, survival, and tissue repair. 

There is evidence supporting the use of EGF in helping wound healing and treating some severe skin conditions.^[11]Shin, S. H., Koh, Y. G., Lee, W. G., Seok, J., & Park, K. Y. (2023). The use of epidermal growth factor in dermatological practice. International Wound Journal. 20(6). 2414-2423. Available at: … Continue reading 

However, a review of trials exploring EGF for anti-aging skin therapy found the evidence was inconclusive, and many studies were poorly controlled.^[12]Miller-Kobisher, B., Suárez-Vega, D. V., & Velazco de Maldonado, G. J. (2021). Epidermal growth factor in aesthetics and regenerative medicine: systematic review. Journal of Cutaneous and … Continue reading

Some evidence from animal models led one research group to suggest that EGF could act as a biologic ‘switch’ at the start and end of the anagen phase of the hair follicle cycle, therefore playing an important role in hair growth.^[13]Mak, K. K. L., & Chan, S. Y. (2003). Epidermal growth factor as a biologic switch in hair growth cycle. Journal of Biological Chemistry. 278(28). 26120-26126. Available at: … Continue reading 

One case report suggested that EGF, used to aid in wound repair, resulted in hair growth on an area of the skin where no hair had previously been seen. However, a study of 60 individuals with an immune condition called alopecia areata, which causes hair loss, found they had higher levels of EGF than healthy controls.^[14]Hyun, M. Y., Suk, J. M., Yoo, K. H., Kim, B. J., Kim, M. N., & Hong, C. K. (2014). Unwanted hair growth induced by topical epidermal growth factor during wound healing: true or myth?. … Continue reading,^[15]El-Refaey, A. M., Khashaba, R. A., El-Habbak, D. M., & Al Husseni, R. M. (2020). Evaluation of serum levels of epidermal growth factor (EGF) in patients with alopecia areata. Benha Journal of … Continue reading

The role of EGF in hair growth is, therefore, complex, and there is no reliable evidence supporting its use to promote hair health or hair growth. Importantly, there are no preclinical studies that demonstrate the biological activity of sh-oligopeptide-1 in cellular or animal models, so it is not possible to predict its action based on studies using EGF.^[17]Martínez-Carpio, P. A. (2023). Topical application of sh-oligopeptide-1 and clinical trials with cosmetic preparations: risk or fraud?. Cutaneous and Ocular Toxicology. 42(4). 190-197. Available at: … Continue reading

SH-Polypeptide-4

This synthetic peptide is an analog of stem cell factor (SCF), a signaling molecule that can regulate stem cell activity. SCF is involved in hair follicle biology, and dysregulation of SCF activity can contribute to hair loss, although it is not usually considered a primary driver like DHT or inflammation.^[18]Randall, V. A., Jenner, T. J., Hibberts, N. A., De Oliveira, I. O., & Vafaee, T. (2008). Stem cell factor/c-Kit signalling in normal and androgenetic alopecia hair follicles. *Journal of … Continue reading

SCF has been studied mechanistically via its receptor c‑KIT. One observational study in androgenic alopecia (AGA) showed altered c‑KIT/SCF signaling in follicles: c‑KIT is upregulated in alopecic follicles, likely to compensate for reduced SCF. This correlated with shorter follicles and reduced stem cell proliferation, and is consistent with suppressed hair growth.^[19]Ashrafuzzaman, M., Yamamoto, T., Shibata, N., Hirayama, T. T., & Kobayashi, M. (2010). Potential involvement of the stem cell factor receptor c-kit in alopecia areata and androgenetic alopecia: … Continue reading

A related study on SCF/c‑KIT signaling in normal vs AGA follicles suggested that androgens reduce dermal papilla SCF, which in turn stops melanocyte function and contributes to depigmented, miniaturized hairs.^[20]Randall, V. A., Jenner, T. J., Hibberts, N. A., De Oliveira, I. O., & Vafaee, T. (2008). Stem cell factor/c-Kit signalling in normal and androgenetic alopecia hair follicles. *Journal of … Continue reading

Importantly, these are mechanistic or associational, not interventional, and no peer‑reviewed human clinical trials are using SCF alone as a hair loss treatment.

SH-Polypeptide-7

As we’ve seen with the other synthetic peptides above, the evidence for sh-Polypeptide-7 is mostly from manufacturer data and multi‑factor formulations rather than standalone, peer‑reviewed clinical trials. Sh‑polypeptide‑7 is a fragment of human growth hormone (hGH, also known as somatotropin). As with the SCF-mimicking sh-polypeptide-4, the proposed activity of hGH is related to its impact on stem cells. It is marketed to stimulate cell growth, regeneration, and “stem‑cell–like” activity in skin and scalp, and is included in several hair‑growth cosmetic serums and liposomal complexes. 

Peer‑reviewed clinical trials where sh‑polypeptide‑7 is isolated as the sole active and rigorously tested for hair‑loss conditions are lacking. Most “clinically proven” language refers to multi‑peptide cocktails in which the specific contribution of sh‑polypeptide‑7 cannot be determined, and the peptide is included in the same QR 678 multi-factor formulation as sh-oligopeptide-4.^[21]Kapoor, R., Shome, D., Vadera, S., & Ram, M. S. (2020). QR 678 & QR678 Neo vs PRP—A randomized, comparative, prospective study. Journal of Cosmetic Dermatology. 19(11). 2877-2885. Available … Continue reading

SH-Polypeptide-9

Sh-polypeptide-9 is designed to mimic vascular endothelial growth factor (VEGF), a signaling molecule that plays an important role in the formation of blood vessels, also known as angiogenesis. 

A laboratory study that grew hair follicle cells alongside blood vessel-like cells suggested that sh-polypeptide-9 could support angiogenesis and enhance pro-follicle signaling. Angiogenesis is fundamental to initiating and maintaining hair growth because the follicle growth phase depends on a dynamic blood flow.^[22]Bassino, E., Zanardi, A., Gasparri, F., & Munaron, L. M. (2016). Effects of the biomimetic peptide sh-polipeptide-9 (CG-VEGF) on cocultures of human hair follicle dermal papilla cells and … Continue reading

Increased angiogenesis is one mechanism behind minoxidil’s effective use against hair loss, and the drug has been shown to increase levels of VEGF in the blood. The role of VEGF in follicle development is well understood, and blocking its activity in animal models can prevent hair growth.^[23]Mecklenburg, L., Tobin, D. J., Müller-Röver, S., Handjiski, B., Wendt, G., Peters, E. M. J., Pohl, S., Moll, I., & Paus, R. (2000). Active hair growth (anagen) is associated with angiogenesis. … Continue reading,^[24]Yano, K., Brown, L. F., & Detmar, M. (2001). Control of hair growth and follicle size by VEGF-mediated angiogenesis. *The Journal of Clinical Investigation.* 107(4). 409–417. Available at: … Continue reading

Again, however, clinical data are lacking. One study showed promising results using a microneedling approach to deliver VEGF alongside the hair loss drug ritlecitinib (brand name Litfulo™). However, no studies have investigated topical VEGF or biomimetic peptides alone.^[26]Ding, Y. W., Li, Y., Zhang, Z. W., Dao, J. W., & Wei, D. X. (2024). Hydrogel forming microneedles loaded with VEGF and Ritlecitinib/polyhydroxyalkanoates nanoparticles for mini-invasive … Continue reading

Overall, there is limited evidence supporting the use of peptides to improve hair growth. While there are often sound theories underpinning their use there is minimal clinical evidence to support the use of these synthetic peptides, or their natural counterparts, as anti-aging or longevity supplements in the hair.

Next, we’ll look at some of the other active ingredients.

Nicotinamide Mononucleotide

Nicotinamide mononucleotide (NMN) is sold mainly as a stand‑alone “longevity” ingredient or in broader supplement mixtures that aim to support energy levels and anti-aging. NMN is a precursor to nicotinamide adenine dinucluotide (NAD+), which is essential for mitochondrial energy production and has been linked to stem cell activity. Aging and DHT both reduce NAD⁺ and drive oxidative stress, inflammation, and stem cell exhaustion, all of which contribute to follicle miniaturization and shorter anagen phases.

Preclinical data published in 2024 showed that topical NMN reversed DHT‑induced hair follicle atrophy, thinning, and sparsity, with regrowth quality similar to minoxidil in a mouse model.^[27]Xu, C., Dai, J., Ai, H., Du, W., & Ji, H. (2024). β-Nicotinamide mononucleotide promotes cell proliferation and hair growth by reducing oxidative stress. Molecules. 29(4). 798. Available at: … Continue reading

A recent small-scale clinical study looked at the impact of oral NMN on hair growth. The study used a diagnostic tool, TrichoScan, to assess participants’ hair growth before and after NMN treatment.^[28]Fukumoto, S., Ito, M., Kunitomo, H., Hataoka, T., Chiba, T., Nureki, O., & Fujimoto, T. (2025). Oral supplementation of nicotinamide mononucleotide (NMN) improves hair quality and subjective … Continue reading 

They found that following NMN treatment, the cellular growth phase was longer, and hair diameter increased. Importantly, however, the study was not controlled: there was only one group observed before and after treatment. Furthermore, the participants did not have hair loss conditions, although they did report issues with hair thinning. Alongside the small size of the study (15 participants), the lack of conventional measures such as terminal hair density, and the use of oral, rather than topical, NMN, it is not possible to draw any conclusions about the effectiveness of NMN from this research.^[29]Fukumoto, S., Ito, M., Kunitomo, H., Hataoka, T., Chiba, T., Nureki, O., & Fujimoto, T. (2025). Oral supplementation of nicotinamide mononucleotide (NMN) improves hair quality and subjective … Continue reading

NMN has been the subject of extensive research into its potential anti-aging benefits as a supplement. However, robust clinical data are required before hair regeneration can be added to its list of potential benefits.

Curcumin

Curcumin, a polyphenolic compound derived from turmeric, is widely studied for its antioxidant and anti-inflammatory properties. These mechanistic qualities often inspire claims that curcumin can support hair growth or counteract pathways implicated in AGA.

In in vitro (cell-based) studies, curcumin has been shown to reduce inflammatory molecules that are also involved in hair loss disorders. Some prostate cell and rodent studies also indicate it may alter androgen metabolism by reducing DHT and increasing AKR1C2, a key protein involved in androgen metabolism.^[30]Ide, H., Lu, Y., Noguchi, T., Muto, S., Okada, H., Kawato, S., & Horie, S. (2018). Modulation of AKR1C2 by curcumin decreases testosterone production in prostate cancer. *Cancer Science.* 109(4). … Continue reading

 

For hair, the data are weak. One small mouse study in a stress-induced alopecia model found that oral curcumin partially improved markers of anagen and hair density but did not restore them to normal, and there are no human clinical trials of curcumin alone for hair loss, either oral or topical. Curcumin often appears in multi-ingredient hair supplements, but its independent contribution is unclear.^[32]Vyas, N., Keservani, R. K., Nayak, A., Jain, S., & Singhal, M. (2010). Effect of *Tamarindus indica* and *Curcuma longa* on stress-induced alopecia. *Pharmacologyonline.* 1. 377–384. Available … Continue reading

Copper Tripeptide-1

Copper peptides, which combine amino acids with copper, occur naturally in the human body. Also known as GHK-Cu, copper tripeptide is commonly used in skincare products. The perfect hair health evidence quality score for GHK-Cu is only 2%, indicating minimal evidence supporting its use against hair loss.

In vitro studies have shown that copper peptides can have anti-inflammatory properties and can enhance the expression of growth factors like VEGF in dermal papilla cells.^[33]Gruchlik, A., Jurzak, M., Chodurek, E. W. A., & Dzierzewicz, Z. (2012). Effect of Gly-Gly-His, Gly-His-Lys and their copper complexes on TNF-alpha-dependent IL-6 secretion in normal human dermal … Continue reading

One small-scale trial of 45 individuals with male pattern hair loss assessed the impact of GHK-Cu in a complex with another amino acid. They found no difference in hair length and hair thickness after 6 months.^[34]Lee, W. J., Sim, H. B., Jang, Y. H., Lee, S. J., Kim, D. W., & Yim, S. H. (2016). Efficacy of a complex of 5-aminolevulinic acid and glycyl-histidyl-lysine peptide on hair growth. *Annals of … Continue reading

Caffeine

Caffeine is one of the more evidence-supported ingredients in the Blueprint Haircare Stack, but its benefits are modest. Topical caffeine has been shown in several small studies to reduce hair shedding and increase the anagen phase in men with AGA. Mechanistically, caffeine can penetrate follicles, mildly increase microcapillary blood flow, and stimulate keratinocyte activity, effects that may help slow miniaturization.^[36]Dressler, C., Blumeyer, A., Rosumeck, S., Arayesh, A., & Nast, A. (2017). Efficacy of topical caffeine in male androgenetic alopecia. *JDDG: Journal der Deutschen Dermatologischen Gesellschaft.* … Continue reading

However, the clinical evidence is of low quality. Most studies use weak endpoints (like hair-pull tests), combine caffeine with minoxidil or azelaic acid, or lack placebo controls. Only one study tested caffeine alone, and even then, it compared caffeine to minoxidil rather than to a true control. As a result, no reliable data shows that caffeine can meaningfully increase hair counts or reverse established thinning.^[37]Dhurat, R., Chitallia, J., May, T. W., Jayaraaman, A. M., Madhukara, J., Anandan, S., Vaidya, P., & Klenk, A. (2017). An open-label randomized multicenter study assessing the noninferiority of a … Continue reading

Caffeine is safe, inexpensive, and may provide minor improvements when used consistently, especially in leave-on formulations around 0.2% concentration. In the context of Blueprint’s serum and shampoo, caffeine is a supportive but low-impact ingredient.

You can read more about the effectiveness of caffeine for hair loss in our in-depth article here.

Adenosine

Adenosine is a well-known cosmetic ingredient with more supportive data than many peptides in the Blueprint stack, though its effects remain modest. In vitro and animal studies show that adenosine can upregulate growth factors involved in follicle development, activate pathways like Wnt/β-catenin, and extend the anagen phase. Ex vivo studies (using tissues outside the body) on human follicles have also demonstrated longer anagen duration and inhibition of androgen signaling.^[38]Hwang, K.-A., Hwang, Y.-L., Lee, M.-H., Kim, N.-R., Roh, S.-S., Lee, Y., Kim, C.-D., & Choi, K.-C. (2012). Adenosine stimulates growth of dermal papilla and lengthens the anagen phase by … Continue reading,^[39]Kim, J., Shin, J. Y., Choi, Y.-H., Joo, J. H., Kwack, M. H., Sung, Y. K., & Kang, N. G. (2024). Hair thickness growth effect of adenosine complex in male-/female-patterned hair loss via … Continue reading

Clinical studies suggest that while adenosine is unlikely to treat hair loss or increase hair counts meaningfully, it may modestly improve hair thickness or fiber diameter over longer periods (around 12 months). One small trial combining caffeine and adenosine showed increases in density and reduced shedding. Still, the contribution of each ingredient is unclear.^[40]Iwabuchi, T., Ideta, R., Ehama, R., Yamanishi, H., Iino, M., Nakazawa, Y., Kobayashi, T., Ohyama, M., & Kishimoto, J. (2016). Topical adenosine increases the proportion of thick hair in Caucasian … Continue reading

A recent systematic review and meta-analysis concluded that the evidence remains low due to flawed designs and small sample sizes in most trials.^[41]Szendzielorz, E., & Spiewak, R. (2025). Adenosine as an Active Ingredient in Topical Preparations Against Hair Loss: A Systematic Review and Meta-Analysis of Published Clinical Trials. … Continue reading

Niacinamide

Niacinamide is a common cosmetic ingredient used for supporting skin barrier function and providing antioxidant effects. In vitro and ex vivo studies suggest it may help protect follicular cells from oxidative stress and extend the growth phase, theoretically supporting longer growth-phase duration. However, other lab studies report no direct stimulation of follicle growth pathways, noting that niacinamide does not increase VEGF.^[42]Oblong, J. E., Peplow, A. W., Hartman, S. M., & Davis, M. G. (2020). Topical niacinamide does not stimulate hair growth based on the existing body of evidence. *International Journal of Cosmetic … Continue reading

Clinical evidence is limited and not particularly compelling. Some small studies report improvements in hair thickness or subjective fullness, especially in women, but these outcomes were not measured with robust or quantifiable methods. Importantly, no trials show that niacinamide increases hair count or reverses miniaturization.^[43]Draelos, Z. D., Jacobson, E. L., Kim, H., Kim, M., & Jacobson, M. K. (2005). A pilot study evaluating the efficacy of topically applied niacin derivatives for treatment of female pattern … Continue reading

What’s Missing Matters

While Blueprint’s serum and shampoo lean heavily on an impressive list of synthetic peptides and longevity-inspired ingredients (such as NMN, curcumin, and copper tripeptide), the formulation is equally defined by what it doesn’t include. Several well-studied, dermatologist-recommended ingredients for androgenic hair loss are noticeably absent. Such ingredients, while less flashy than biomimetic peptides, have substantially stronger evidence behind them.

These include plant-based DHT modulators, clinically reviewed botanicals, and cosmetic actives that appear repeatedly in peer-reviewed dermatology literature:

• Saw Palmetto (Serenoa repens): Small human trials and mechanistic studies suggest mild 5-α-reductase inhibition, the same hormonal pathway targeted by finasteride, though far weaker.^[44]Suzuki, M., Ito, Y., Fujino, T., Abe, M., Umegaki, K., Onoue, S., Noguchi, H., & Yamada, S. (2009). Pharmacological effects of saw palmetto extract in the lower urinary tract. *Acta … Continue reading
• Pumpkin Seed Oil:  Some limited studies have demonstrated improvements in hair density or reduced shedding.^[45]Gatti, M., Ippoliti, I., Poluzzi, E., Antonazzo, I. C., Moro, P. A., Moretti, U., Menniti-Ippolito, F., Mazzanti, G., De Ponti, F., & Raschi, E. (2021). Assessment of adverse reactions to … Continue reading
• Topical Rosemary Oil: In at least one randomized trial, rosemary oil demonstrated modest improvements in hair count, comparable to 2% minoxidil.^[46]Panahi, Y., Taghizadeh, M., Marzony, E. T., & Sahebkar, A. (2015). Rosemary oil vs minoxidil 2% for the treatment of androgenetic alopecia: a randomized comparative trial. *Skinmed.* 13(1). … Continue reading
• Melatonin: Topical melatonin may decrease rates of active hair loss for patients with early AGA.^[47]Fischer, T. W., Burmeister, G., Schmidt, H. W., & Elsner, P. (2004). Melatonin increases anagen hair rate in women with androgenetic alopecia or diffuse alopecia: results of a pilot randomized … Continue reading,^[48]Fischer, T. W., Trüeb, R. M., Hänggi, G., Innocenti, M., & Elsner, P. (2012). Topical melatonin for treatment of androgenetic alopecia. *International Journal of Trichology.* 4(4). 236–245. … Continue reading
• Redensyl, Capixyl, and Procapil (RCP): One industry-funded study has shown results from topical RCP similar to those from 5% minoxidil.^[49]Karaca, N., & Akpolat, N. D. (2019). A comparative study between topical 5% minoxidil and topical “Redensyl, Capixyl, and Procapil” combination in men with androgenetic alopecia. *Journal of … Continue reading

By contrast, many of the Blueprint actives (niacinamide, NMN, curcumin derivatives, and most synthetic peptides) have minimal or no clinical evidence supporting their ability to improve human hair growth. They are mechanistically intriguing, often tied to cellular metabolism or regenerative biology, but largely untested on real scalps in real patients.

This contrast raises an important question: Why exclude better-documented natural actives in favor of less-validated, high-concept molecules?

Several explanations make sense in the context of Blueprint’s brand:

• Brand Consistency: Blueprint favors “longevity” molecules associated with aging pathways, mirroring Johnson’s systemic supplement stack rather than traditional dermatologic practice.
• Aesthetic Minimalism: Omitting herbal or “earthy” ingredients maintains a sleek, biotech-related identity, positioning the products nearer to medical-grade skincare than to naturopathic formulations.
• Intellectual Differentiation: A peptide-heavy, techno-sounding ingredient list creates the perception of a proprietary innovation rather than another botanical-based hair serum.

However, this decision appears to require a trade-off between novelty and evidence-based efficacy, and Blueprint’s formulation prioritizes conceptual novelty over empirical strength.

Interestingly, Brian Johnson’s own haircare protocol does not include the ingredients found in the Blueprint Hair Stack. Instead, he relies on clinically validated pharmaceutical solutions, including 7% topical minoxidil and 0.25% topical dutasteride, as well as daily oral minoxidil.^[50]Bryan Johnson. (2024). How I Reversed My Hair Loss. *Blueprint Bryan Johnson.* Available at: … Continue reading

Peptide Shampoo

The shampoo formula repeats many of the peptides and actives found in the serum, though likely at lower concentrations and with brief contact time, limiting their potential impact. Blueprint’s Peptide Shampoo uses mild surfactants such as glucosides and sulfosuccinates, which are gentle on the scalp barrier but unlikely to deliver meaningful penetration of active ingredients. Additives like menthol and silk amino acids offer a pleasant sensory effect, acting to provide cooling, softness, and scalp “refreshment”. However, they provide little evidence-backed benefit for long-term hair health. Overall, the shampoo mirrors the serum’s branding and high-tech positioning but offers minimal functional innovation or clinical value.

The full list of ingredients for the Blueprint Peptide Shampoo is as follows: water, lauryl glucoside, glycerin, decyl glucoside, coco-glucoside, disodium lauryl sulfosuccinate, pentylene glycol, lauramidopropyl betaine, sodium cocoyl apple amino acids, betaine, glycol distearate, polyquarternium-10, caprylyl/capryl glucoside, sh-oligopeptide-1, sh-oligopeptide-4, sh-polypeptide-1, sh-polypeptide-7, sh-polypeptide-9, sh-polypeptide-11, sh-polypeptide-71, copper tripeptide-1 (DHK-Cu), niacinamide, 3-0-ethyl ascorbic acid, adenosine, caddeine, arginine, tetrahydrocurcumin, curcumin, menthol, silk amino acids, tetrasodium glutamate diacetate, mentha arvensis herb oil, lecithin, trehalose, glyceryl caprylate, hexylene glycol, panthenol, polysorbate 60, citric acid, sodium citrate, sodium chloride, phenoxyethanol, limonene.

Is the Haircare Stack Safe?

Blueprint’s serum and shampoo appear broadly safe for cosmetic use. The surfactant system in the shampoo is relatively mild (glucosides, sulfosuccinates), which is generally positive for barrier preservation. Similarly, most ingredients (peptides, NMN, niacinamide, caffeine, adenosine, copper peptides, curcumin derivatives) are already used in cosmetic or dermatologic products without major safety signals at typical concentrations.

However, some ingredients may cause irritation. Salicylic acid is a well‑known keratolytic that can cause stinging, dryness, peeling, and dermatitis, particularly on sensitive or compromised skin. Curcumin, despite its anti‑inflammatory properties, is a documented contact allergen in some users, with cases of allergic contact dermatitis reported. Thioctic acid can also produce burning and erythema in reactive skin.^[52]Brumley, C., Arora, P., Neeley, A., & Hylwa, S. (2024). Allergic contact dermatitis to salicylic acid: A case series of relevant sensitization. *Contact Dermatitis.* 91(2). 133–138. Available … Continue reading,^[53]Chaudhari, S. P., Tam, A. Y., & Barr, J. A. (2015). Curcumin: a contact allergen. *The Journal of Clinical and Aesthetic Dermatology.* 8(11). 43–48. Available at: … Continue reading,^[54]Gatti, M., Ippoliti, I., Poluzzi, E., Antonazzo, I. C., Moro, P. A., Moretti, U., Menniti-Ippolito, F., Mazzanti, G., De Ponti, F., & Raschi, E. (2021). Assessment of adverse reactions to … Continue reading

Should I Use It?

Due to a lack of supportive evidence from clinical studies, Blueprint Hair Stack isn’t recommended if you have established AGA and are seeking maximal, cost-effective regrowth. The absence of clinically validated ingredients, either natural or pharmaceutical, is a major limitation. 

The stack might be a good fit if you appreciate and buy into Bryan Johnson’s longevity narrative and are comfortable paying a premium for branding, aesthetics, and mechanistic novelty. The product may improve cosmetic scalp and hair quality (softness, shine) rather than promote regrowth.

Recommendations to the Company

From a formulation perspective, introducing a version that includes at least one well-validated active (e.g., evidence-based botanicals like saw palmetto or rosemary) would make the product more appealing to those seeking real regrowth.

Blueprint could significantly strengthen the credibility of its Haircare Stack by generating product-specific clinical data and being more transparent about formulation details. Even a small randomized, controlled trial using objective endpoints would go a long way toward substantiating “science-backed” claims. Publishing the concentrations of key ingredients would also help clinicians and informed consumers meaningfully assess the formula.

Final Thoughts

Blueprint markets its Haircare Stack as “hair longevity rooted in science.” At the ingredient level, there are often theoretical foundations for the selection of synthetic peptides and actives used in serum and shampoo.

However, when we look at the evidence for outcomes that matter most to people with hair thinning and hair loss, the picture is less compelling. There are few components backed by clinical studies, and a noticeable absence of the most strongly validated treatments and well-studied natural ingredients.

In its current form, the Blueprint Haircare Stack functions more as a longevity-themed, cosmetically elegant scalp serum and shampoo than as a robust hair regrowth therapy. For consumers who resonate with Bryan Johnson’s philosophy and can comfortably afford the price, it may be an enjoyable, “on-brand” part of a broader routine. For those primarily seeking maximum regrowth per dollar, existing evidence suggests that more traditional, clinically tested options remain the stronger choice.